There It Is!

Album3 (DVD): Blues Clues: Blue’s Big Musical Movie, Paramount, 3 October 2000.
Album3 (CD + Book): Rhino/WEA, 17 October 2000. I’ve also seen VHS and Playstation versions.

Performed by Ray Charles (in his audio-only role as G-Clef) and The Persuasions.

HQ audio, album version:

(Video + LQ audio:)